解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one, two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and from these, infor...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Primate pericentromeric regions recently have been shown to exhibit extraordinary evolutionary plasticity. In this paper we report an additional peculiar feature of these regions that we discovered while analyzing, by FISH, the evolutionary conservation of primate phylogenetic chromosome IX. If the position of the cen...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.9.12.1184
更新日期:1999-12-01 00:00:00
abstract::Double anal fin (Da) is a medaka with an autosomal semidominant mutation that causes mirror image duplication of the ventral region concentrating on the caudal region. The chromosomal location of the Da gene and its sequence have remained unknown. We constructed a medaka linkage map as a first step to approach positio...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.9.12.1277
更新日期:1999-12-01 00:00:00
abstract::Analysis procedures are needed to extract useful information from the large amount of gene expression data that is becoming available. This work describes a set of analytical tools and their application to yeast cell cycle data. The components of our approach are (1) a similarity measure that reduces the number of fal...
journal_title:Genome research
pub_type: 杂志文章,评审
doi:10.1101/gr.9.11.1106
更新日期:1999-11-01 00:00:00
abstract::Previously, we have described novel families of genes, warthog (wrt) and groundhog (grd), in Caenorhabditis elegans. They are related to Hedgehog (Hh) through the carboxy-terminal autoprocessing domain (called Hog or Hint). A comprehensive survey revealed 10 genes with Hog/Hint modules in C. elegans. Five of these are...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.9.10.909
更新日期:1999-10-01 00:00:00
abstract::With the genomic sequencing of Arabidopsis nearing completion and rice sequencing very much in its infancy, a key question is whether we can exploit the Arabidopsis sequence to identify candidate genes for traits in cereal crops using a map-based approach. This requires the existence of colinearity between the Arabido...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.9.9.825
更新日期:1999-09-01 00:00:00
abstract::We have established a landmark framework map over 20-25 Mb of the long arm of the human X chromosome using yeast artificial chromosome (YAC) clones. The map has approximately one landmark per 45 kb of DNA and stretches from DXS7531 in proximal Xq23 to DXS895 in proximal Xq26, connecting to published framework maps on ...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Comparative analysis of the protein sequences encoded in the four euryarchaeal species whose genomes have been sequenced completely (Methanococcus jannaschii, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, and Pyrococcus horikoshii) revealed 1326 orthologous sets, of which 543 are represented in all fou...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Using both env and long terminal repeat (LTR) sequences, with maximal representation of genetic diversity within primate strains, we revise and expand the unique evolutionary history of human and simian T-cell leukemia/lymphotropic viruses (HTLV/STLV). Based on the robust application of three different phylogenetic al...
journal_title:Genome research
pub_type: 杂志文章,评审
doi:
更新日期:1999-06-01 00:00:00
abstract::We have performed detrended DNA walks on whole prokaryotic genomes, on noncoding sequences and, separately, on each position in codons of coding sequences. Our method enables us to distinguish between the mutational pressure associated with replication and the mutational pressure associated with transcription and othe...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Single-nucleotide polymorphisms (SNPs) are the most frequently found DNA sequence variations in the human genome. It has been argued that a dense set of SNP markers can be used to identify genetic factors associated with complex disease traits. Because all high-throughput genotyping methods require precise sequence kn...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Vaccinia DNA topoisom...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Interferons and interleukin-10 are involved in key aspects of the host defence mechanisms. Human chromosome 21 harbors the interferon/interleukin-10 receptor gene cluster linked to the GART gene. This cluster includes both components of the interferon alpha/beta-receptor (IFNAR1 and IFNAR2) and the second components o...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Mutations in the human AIRE gene (hAIRE) result in the development of an autoimmune disease named APECED (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy; OMIM 240300). Previously, we have cloned hAIRE and shown that it codes for a putative transcription-associated factor. Here we report the cloning and...
journal_title:Genome research
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::Hearing impairment is clinically and genetically heterogeneous. There are >400 disorders in which hearing impairment is a characteristic of the syndrome, and family studies demonstrate that there are at least 30 autosomal loci for nonsyndromic hearing impairment. The genes that have been identified encode diaphanous (...
journal_title:Genome research
pub_type: 历史文章,杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::Mutational analysis of large genes with complex genomic structures plays an important role in medical genetics. Technical limitations associated with current mutation screening protocols have placed increased emphasis on the development of new technologies to simplify these procedures. High-density arrays of >90,000-o...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.12.1245
更新日期:1998-12-01 00:00:00
abstract::We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequili...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.11.1172
更新日期:1998-11-01 00:00:00
abstract::The prion protein (PrP), first identified in scrapie-infected rodents, is encoded by a single exon of a single-copy chromosomal gene. In addition to the protein-coding exon, PrP genes in mammals contain one or two 5'-noncoding exons. To learn more about the genomic organization of regions surrounding the PrP exons, we...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.10.1022
更新日期:1998-10-01 00:00:00
abstract::A total of 202 genes were cytogenetically mapped to goat chromosomes, multiplying by five the total number of regional gene localizations in domestic ruminants (255). This map encompasses 249 and 173 common anchor loci regularly spaced along human and murine chromosomes, respectively, which makes it possible to perfor...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.9.901
更新日期:1998-09-01 00:00:00
abstract::The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.8.842
更新日期:1998-08-01 00:00:00
abstract::Comparative gene mapping in mammals typically involves identification of segments of conserved synteny in diverse genomes. The development of maps that permit comparison of gene order within conserved synteny has not advanced beyond the mouse map that takes advantage of linkage analysis in interspecific backcrosses. R...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.7.731
更新日期:1998-07-01 00:00:00
abstract::In contrast to other animal cell lines, the chicken pre-B cell lymphoma line, DT40, exhibits a high level of homologous recombination, which can be exploited to generate site-specific alterations in defined target genes or regions. In addition, the ability to generate human/chicken monochromosomal hybrids in the DT40 ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.6.666
更新日期:1998-06-01 00:00:00
abstract::The str family of genes encoding seven-transmembrane G-protein-coupled or serpentine receptors related to the ODR-10 diacetyl chemoreceptor is very large, with at least 197 members in the Caenorhabditis elegans genome. The closely related stl family has 43 genes, and both families are distantly related to the srd fami...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.5.449
更新日期:1998-05-01 00:00:00
abstract::As the Human Genome Project moves into its sequencing phase, a serious problem has arisen. The same problem has been increasingly vexing in the closing phase of the Caenorhabditis elegans project. The difficulty lies in sequencing efficiently through certain regions in which the templates (DNA substrates for the seque...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.5.562
更新日期:1998-05-01 00:00:00
abstract::We have identified three new families of insulin homologs in Caenorhabditis elegans. In two of these families, concerted mutations suggest that an additional disulfide bond links B and A domains, and that the A-domain internal disulfide bond is substituted by a hydrophobic interaction. Homology modeling remarkably con...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.4.348
更新日期:1998-04-01 00:00:00
abstract::Large numbers of expressed sequence tags (ESTs) continue to fill public and private databases with partial cDNA sequences. However, using this huge amount of ESTs to facilitate gene finding in genomic sequence imposes a challenge, especially to wet-lab scientists who often have limited computing resources. In an effor...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.3.268
更新日期:1998-03-01 00:00:00
abstract::Diversity in the antigen-binding receptors of the immune system has long been a primary interest of biologists. Recently it has been suggested that polymorphism in regulatory (noncoding) gene segments is of substantial importance as well. Here, we survey the level of variation in MHC class II gene promoters in man and...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.8.2.124
更新日期:1998-02-01 00:00:00
abstract::We have assembled a first-generation anchor map of the mouse genome using a panel of 94 whole-genome-radiation hybrids (WG-RHs) and 271 sequence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.12.1153
更新日期:1997-12-01 00:00:00
abstract::The genetic positions of the five Arabidopsis thaliana centromere regions have been identified by mapping size polymorphisms in the centromeric 180-bp repeat arrays. Structural and genetic analysis indicates that 180-bp repeat arrays of up to 1000 kb are found in the centromere region of each chromosome. The genetic b...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.11.1045
更新日期:1997-11-01 00:00:00
abstract::Y chromosome haplotypes are particularly useful in deciphering human evolutionary history because they accentuate the effects of drift, migration, and range expansion. Significant acceleration of Y biallelic marker discovery and subsequent typing involving heteroduplex detection has been achieved by implementing an in...
journal_title:Genome research
pub_type: 信件
doi:10.1101/gr.7.10.996
更新日期:1997-10-01 00:00:00
abstract::The association of subclasses of Alu repetitive elements with various classes of trinucleotide and tetranucleotide microsatellites was characterized as a first step toward advancing our understanding of the evolution of microsatellite repeats. In addition, information regarding the association of specific classes of m...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.7.716
更新日期:1997-07-01 00:00:00
abstract::We report the construction of a dense linkage map of the rat genome integrating 767 simple sequence length polymorphism markers, combined over three crosses with high rates of polymorphism. F2 populations from WKY x S (n = 159), BN x S (n = 91), and BN x GK (n = 139) were selected and genotyped for combinations of mic...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.5.434
更新日期:1997-05-01 00:00:00
abstract::Repetitive DNA is a significant component of eukaryotic genomes. We have developed a strategy to efficiently and accurately sequence repetitive DNA in the nematode Caenorhabditis elegans using integrated artificial transposons and automated fluorescent sequencing. Mapping and assembly tools represent important compone...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.5.551
更新日期:1997-05-01 00:00:00
abstract::Forty-three yeast artificial chromosomes (YACs) from the X chromosome have been overlapped across the 4-Mb Xq21.3 region, which is homologous to a segment in Yp11.1. The region is formatted to 60-kb resolution with 57 STSs and is merged at its edges with contigs specific for X. This allows a direct comparison of marke...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.4.307
更新日期:1997-04-01 00:00:00
abstract::The detailed genomic organization of a gene-dense region at human chromosome 12p13, spanning 223 kb of contiguous sequence, was determined. This region is composed of 20 genes and several other expressed sequences. Experimental tools including RT-PCR and cDNA sequencing, combined with gene prediction programs, were ut...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.3.268
更新日期:1997-03-01 00:00:00
abstract::Chromosome-specific cDNA libraries are new tools for the isolation of genes from specific genomic regions. We have used two YACs than span the approximately 2-Mb cri-du-chat critical region (CDCCR) of chromosome 5p to directly screen a chromosome 5-specific (CH5SP) fetal brain cDNA library. To compare this library wit...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.2.118
更新日期:1997-02-01 00:00:00
abstract::Giardia duodenalis is the best-characterized example of the most ancient eukaryotes, which are primitively amitochondrial and anaerobic. The surface of Giardia is coated with cysteine-rich proteins. One family of these proteins, CRP136, varies among isolates and upon environmental stress. A repeat region within the CR...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.7.1.37
更新日期:1997-01-01 00:00:00
abstract::The genome size of Pseudoalteromonas haloplanktis, a ubiquitous and easily cultured marine bacterium, was measured as a step toward estimating the genome complexity of marine bacterioplankton. To determine total genome size, we digested P. haloplanktis DNA with the restriction endonucleases Notl and Sfil, separated th...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.6.12.1160
更新日期:1996-12-01 00:00:00
abstract::We have identified previously a putative tumor suppressor gene (TSG) locus at human chromosome (hchr) 7q31 showing that it is altered in a variety of human epithelial tumors. To determine whether this TSG is conserved in mice, we studied loss of heterozygosity (LOH) in chemically induced mouse liver adenomas. The LOH ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.6.11.1070
更新日期:1996-11-01 00:00:00
abstract::In positional cloning the initial assignment of a gene to a specific chromosomal locus is followed by physical mapping of the critical region. The construction of a high-resolution physical map still involves considerable effort. However, new high-resolution fluorescence in situ hybridization (FISH) techniques have fa...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.6.10.1002
更新日期:1996-10-01 00:00:00